Fig. 5. ICV injection of AChRα7 siRNA for 5 days decreased POMC expression in the hypothalamus. Protein content of (A) CHRNα7 in the hypothalamus of male mice after injection of sc-siRNA control solution for 5 days, or injection of AChRα7 siRNA for 3 days and 5 days (n = 3 for all groups). Expression of mRNA in the hypothalamus of mice after 5 days of injection of AChRα7 siRNA (B) mRNA Chrna7 mRNA POMC (C) AgRP (D) NPY (scRNA-A n = 4, AChRα7 siRNA n = 7). For the evaluation of gene expression, ACTB was used as the endogenous control. In panel A, one-way ANOVA was used. In B, C and D Student's t-test was used ** p <0.01. HYPO=Hypothalamus.